The SPDYA Antibody from MyBioSource.com is a Rabbit Polyclonal antibody to SPDYA. This antibody recognizes Human, Mouse, and Rat antigen. The SPDYA Antibody has been validated for the following applications: ELISA, Immunohistochemistry, and Western Blot.
Description
Description: Speedy A, also known as SPDYA, SPDY1, Ringo3 or SPY1, is a 313 amino acid protein that localizes to the nucleus and belongs to the speedy/ringo family. Expressed at high levels in testis and at lower levels in brain, kidney, heart, bone marrow, colon, lung, liver spleen and placenta, Speedy A functions to regulate the G1/S phase transition of the cell cycle, specifically by binding to and activating Cdc2 p34, Cdk2 and p27. Additionally, Speedy A mediates cell survival during DNA damage, suggesting that Speedy A plays a role in proper cell cycle progression throughout the life of the cell. Multiple isoforms of Speedy A exist due to alternative splicing events. The gene encoding Speedy A maps to human chromosome 2, which encodes over 1, 400 genes and comprises nearly 8% of the human genome.
Function: Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2 (PubMed:12972555). Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation (PubMed:28666995). Mediates cell survival during the DNA damage process through activation of CDK2 (PubMed:12839962).
Subunit Structure: Interacts with CDK1 (By similarity). Interacts with CDK2 (PubMed:11980914, PubMed:12839962, PubMed:12972555, PubMed:28666995). May interact with CDKN1B/KIP1 (PubMed:12972555). Identified in a complex with CDK2 and CDKN1B/KIP1, where it interacts primarily with CDK2 (PubMed:12972555, PubMed:28666995).
Similarity: The C-terminus is required for CDK2-activation, but not CDK2-binding. Belongs to the Speedy/Ringo family